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Registros recuperados: 46
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Genetic diversity associated with agronomic traits using microsatellite markers in Pakistani rice landraces Electron. J. Biotechnol.
Pervaiz,Zahida Hassan; Rabbani,M. Ashiq; Khaliq,Ishtiaq; Pearce,Stephen R; Malik,Salman A.
Genetic diversity underlies the improvement of crops by plant breeding. Land races of rice (Oryza sativa L.) can contain some valuable alleles not common in modern germplasm. The aim here was to measure genetic diversity and its effect on agronomic traits among rice land-race genotypes grown in Pakistan. Diversity was measured using thirty-five microsatellite markers and seventy-five genotypes. Among the markers used a total of 142 alleles were detected at 32 polymorphic SSR loci, while three loci were monomorphic in Pakistani rice landraces. The number of alleles identified by each marker ranged from 2 to 13 with a mean of 4.4. Size differences between the smallest and largest alleles varied from 11bp to 71bp. Polymorphism information content ranged from...
Tipo: Journal article Palavras-chave: Genetic diversity; Landraces; Microsatellite markers; Oryza sativa L.; Pakistan; Rice.
Ano: 2010 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582010000300004
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Genetic impact of the reproduction dynamics in the European flat oyster Ostrea edulis ArchiMer
Lapegue, Sylvie; Taris, Nicolas; Lallias, Delphine; Bonhomme, Francois; Boudry, Pierre.
The European flat oyster (Ostrea edulis L.) is a marine bivalve whose natural geographical distribution ranges along the European Atlantic coast from Norway to Morocco, in addition to the Mediterranean and Black Sea. The latest results obtained on the genetic differentiation between these populations have led us to persue studies at a finer scale, in order to estimate the effective number of breeders and the temporal dynamics of reproduction and, more specially, recruitment. Several experiments were performed to document (1) the variance in allele frequencies during a natural settlement period, (2) the paternal contribution to fertilization by analyzing larvae sampled at the brooding stage within individual females, (3) the variance of individual...
Tipo: Text Palavras-chave: Recruitment; Microsatellite markers; Ostrea edulis; European flat oyster; Genetic.
Ano: 2004 URL: http://archimer.ifremer.fr/doc/2004/acte-3485.pdf
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Genetic improvement of hatchery propagated bivalve stocks : prospects and constraints ArchiMer
Boudry, Pierre.
To date, the most significant genetic improvement for the production of Pacific oyster (Crassostrea gigas) has been obtained through the production of triploids, especially since the development of tetraploids. Quantitative genetics studies suggest that significant gains, for disease resistance or other traits, could be obtained in diploids. However, the limited extent of hatchery-propagation (versus natural recruitment) and/or various technical difficulties and biological characteristics of the species have retarded the development of selective breeding programs. In the U.S.A., Australia and New Zealand, family-based selective breeding programs have recently been initiated to improve growth, disease tolerance and yield. In Europe, where both natural and...
Tipo: Text Palavras-chave: Microsatellite markers; Crassostrea gigas; Selective breeding; Triploid; Pacific oyster; Genetic.
Ano: 2005 URL: http://archimer.ifremer.fr/doc/2005/acte-3451.pdf
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Genetic linkage maps of chicken chromosomes 6, 7, 8, 11 and 13 from a Brazilian resource population Scientia Agricola
Ambo,Marcel; Campos,Raquel de Lello Rocha; Moura,Ana Sílvia Meira Tavares; Boschiero,Clarissa; Rosário,Millor Fernandes do; Ledur,Mônica Corrêa; Nones,Kátia; Coutinho,Luiz Lehmann.
A linkage map is essential not only for quantitative trait loci (QTL) mapping, but also for the organization and location of genes along the chromosomes. The present study is part of a project whose major objective is, besides from construction the linkage maps, the whole genome scan for mapping QTL for performance traits in the Brazilian experimental chicken population. Linkage maps of chicken chromosomes 6 to 8, 11 and 13 were constructed based on this population. The population was developed from two generations of crossbreeding between a broiler and a layer line. Fifty-one microsatellite markers were tested, from which 28 were informative: 4, 8, 7, 4 and 5 for chromosomes 6, 7, 8, 11 and 13, respectively. A SNP located in the leptin receptor gene was...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Gallus gallus; Quantitative trait loci (QTL); Genetic maps; Microsatellite markers; Animal breeding.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162008000500001
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Genetic relationships between Cuban and Brazilian buffaloes (Bubalus bubalis) by microsatellite markers Rev Salud Anim.
Martínez Marrero,Nadia; da Silva,Elizabete Cristina; Mitat Valdés,Alina; Ponce,Giselle; Benício da Silva,Regina C.; Paes Barbosa,Severino Benone; Uffo Reinosa,Odalys; Gomes Filho,Manoel Adrião.
Researches on genetic relationships among water buffalo populations are useful in supporting conservation decisions and designing of breeding schemes. The aim of this study was to assess the genetic relationships among Cuban Buffalypso/Carabao hybrids and Murrah and Jaffarabadi Brazilian buffaloes using nine cattle microsatellite markers. During the analysis, four loci were excluded due to the presence of null alleles, stuttering and gametic disequilibrium. Mean numbers of alleles and the heterozygosities observed and expected in the three populations were not significantly different. Heterozygosities found were above 60%. All five loci were in Hardy-Weinberg equilibrium. Wright´s index for estimating inbreeding were negative in the three populations. A...
Tipo: Journal article Palavras-chave: Bubalus bubalis; Buffalypso; Microsatellite markers; Null allele; Genetic relationships.
Ano: 2015 URL: http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S0253-570X2015000300003
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Genetic similarity in a hybrid population of 'Montenegrina' and 'King' mandarins PAB
Brugnara,Eduardo Cesar; Bastianel,Marinês; Weiler,Roberto Luis; Schwarz,Sergio Francisco.
The objective of this work was to confirm the hybrids obtained in plants originated from the crossing between the mandarins Citrus deliciosa 'Montenegrina' and C. nobilis 'King', and to estimate the genetic similarity among hybrids, and between each hybrid and its parents. Twenty‑three pairs of microsatellite primers were tested. Fourteen of these pairs showed polymorphic bands between parents. Primers CCSM 129 and CCSME 52 were sufficient to identify the 12 nucellar clones observed in the studied population. Genetic similarity analysis of the population (hybrids and parents) showed 0.56 average similarity. Besides the 12 clones of 'Montenegrina' identified, 25 hybrids were found of which D18, C32, D06, C05 and D09 are the more similar to 'Montenegrina'.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Citrus deliciosa; Citrus nobilis; Hybrid identification; Microsatellite markers.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2012000800010
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Genetic variability and efficiency of DNA microsatellite markers for paternity testing in horse breeds from the Brazilian Marajó archipelago Genet. Mol. Biol.
Reis,Sávio P.; Gonçalves,Evonnildo C.; Silva,Artur; Schneider,Maria P.C..
In this study, 15 microsatellite DNA loci used in comparative tests by the International Society for Animal Genetics were applied to the evaluation of genetic diversity and management, and the efficiency of paternity testing in Marajoara horses and Puruca ponies from the Marajó Archipelago. Based on the genotyping of 93 animals, mean allelic diversity was estimated as 9.14 and 7.00 for the Marajoara and Puruca breeds, respectively. While these values are similar to those recorded in most European breeds, mean levels of heterozygosity were much lower (Marajoara 49%, Puruca 40%), probably as a result of high levels of inbreeding in the Marajó populations. The mean informative polymorphic content of this 15-marker system was over 50% in both breeds, and was...
Tipo: Info:eu-repo/semantics/other Palavras-chave: Marajoara horses; Puruca ponies; Microsatellite markers; Genetic diversity; Geneaological control.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000100014
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Genetic variability and selective breeding for traits of aquacultural interest in the Pacific oyster (Crassostrea gigas) (slides) ArchiMer
Boudry, Pierre; Degremont, Lionel; Taris, Nicolas; Mccombie, Helen; Haffray, Pierrick; Ernande, Bruno.
To date, the most significant genetic improvement for the production of Pacific oyster (Crassostrea gigas) has been obtained through the breeding of triploids, especially since the development of tetraploids. Quantitative genetics studies suggest that significant gains, for disease resistance or for other traits of aquacultural interest, could be obtained in diploids using this approach. However, the limited extent of hatchery-propagation (versus natural recruitment) and/or various technical difficulties and biological characteristics of the species have retarded the development of selective breeding programs. Recently, in the U.S.A., Australia and New Zealand, countries where hatcheries are a major source of C. gigas juveniles, family-based selective...
Tipo: Text Palavras-chave: Microsatellite markers; Selective breeding; Genetic; Genetic variability; Crassostrea gigas; Pacific oysters.
Ano: 2004 URL: http://archimer.ifremer.fr/doc/2004/acte-3449.pdf
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Génotypage des géniteurs de Lates calcarifer de Tahiti : aide à la domestication raisonnée du Loup Tropical pour la filière tahitienne ArchiMer
Vonau, Vincent; Rouxel, Catherine; Saulnier, Denis; Cochennec-laureau, Nathalie; Nedelec, Georges; Goyard, Emmanuel.
The tropical seabass Lates calcarifer was introduced in Tahiti in 1984 and then was domesticated without other introduction. Three successive generations have been obtained in captivity. Tissue samples of the 38 animaIs which represent the total Tahitian broodstock were preserved in alcohol to be genotyped with four microsatellite markers (Yue et al., 2001). Three of four described markers were successfully transferred to the laboratory of genetics of Tahiti (marker LcaM01, LcaM02, LcaM03), as the revelation technology available locally did not allow the use of LcaM04 in routine. The results show that the genetic diversity of the tahitian broodstock is not equivalent of the one of the species, which could be explained by the small size of the founder...
Tipo: Text Palavras-chave: Lates calcarifer; Marqueurs microsatellites; Reproducteurs; Variabilité génétique; Lates calcarifer; Microsatellite markers; Breeding stock; Genetic diversity.
Ano: 2003 URL: http://archimer.ifremer.fr/doc/00142/25300/23372.pdf
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High connectivity across the fragmented chemosynthetic ecosystems of the deep Atlantic Equatorial Belt: efficient dispersal mechanisms or questionable endemism? ArchiMer
Teixeira, Sara; Olu, Karine; Decker, Carole; Cunha, Regina L.; Fuchs, Sandra; Hourdez, Stephane; Serrao, Ester A.; Arnaud-haond, Sophie.
Chemosynthetic ecosystems are distributed worldwide in fragmented habitats harbouring seemingly highly specialized communities. Yet, shared taxa have been reported from highly distant chemosynthetic communities. These habitats are distributed in distinct biogeographical regions, one of these being the so-called Atlantic Equatorial Belt (AEB). Here, we combined genetic data (COI) from several taxa to assess the possible existence of cryptic or synonymous species and to detect the possible occurrence of contemporary gene flow among populations of chemosynthetic species located on both sides of the Atlantic. Several Evolutionary Significant Units (ESUs) of Alvinocarididae shrimp and Vesicomyidae bivalves were found to be shared across seeps of the AEB. Some...
Tipo: Text Palavras-chave: Atlantic equatorial belt; Chemosynthetic habitats; Deep-sea connectivity; Endemic bivalves; Endemic shrimp; Genetic diversity; Microsatellite markers; Mitochondrial COI gene.
Ano: 2013 URL: http://archimer.ifremer.fr/doc/00157/26789/24950.pdf
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Inheritance and identification of molecular markers associated with spot blotch (Cochliobolus sativus L.) resistance through microsatellites analysis in barley Genet. Mol. Biol.
Kuldeep,Tyagi; Nandan,Rajiv; Kumar,Uttam; Prasad,Lal Chand; Chand,Ramesh; Joshi,Arun Kumar.
Spot blotch resistant (IBON 18) and susceptible (RD 2508) lines were crossed to investigate inheritance of resistance and to identify simple sequence repeats (SSRs) associated with resistance. F1 resistance was intermediate and suggested additive nature of inheritance. Three additive genes was noted in the distribution of F3, F4 and F5 generations. In F6 and F6-7, the quantitative and qualitative approaches also suggested the control of three resistance genes. The parents and the RILs (F6/F6-7) were grown in four environments and spot blotch severity recorded. Forty five SSR primers, specific for chromosomes 1 (7H) and 5 (1H), were applied. Of these, 12 were polymorphic between the parents, and between the resistant and susceptible bulks. Three markers BMS...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Microsatellite markers; Molecular marker; Spot blotch; Cochliobolus sativus; Barley.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000400021
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Inheritance patterns and identification of microsatellite markers linked to the rice blast resistance in BC2F1 population of rice breeding Bragantia
Miah,Gous; Rafii,Mohd Y.; Ismail,Mohd Razi; Puteh,Adam Bin; Rahim,Harun Abdul; Ashkani,Sadegh; Latif,Abdul.
The BC2F1 population was derived from a cross between rice variety, MR219 (susceptible to blast) and Pongsu Seribu 1 (resistant to blast). The objectives of this research were to know the inheritance pattern of blast resistance and to identify the linked markers associated with blast resistance in BC2F1 population. Sixteen microsatellite markers were found as polymorphic between the parents related to blast resistant genes (Pi-genes). Among the selected blast resistant linked markers, two markers RM6836 and RM8225 showed expected testcross ratio (1:1) for single-gene model in the BC2F1 population with the association between resistant and susceptible progeny. A total of 333-BC2F1 plants were challenged with the most virulent pathotype P7.2 of Magnaporthe...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Blast inheritance; Microsatellite markers; BC2F1 population; Rice variety.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0006-87052015000100033
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Interactions Between Non-Allelic Loci and Their Effects on Categorized Meat Performance Traits in Ducks Rev. Bras. Ciênc. Avic.
Dobek,A; Gornowicz,E; Moliński,IA; Grajewski,B; Lisowski,M; Szwaczkowski,T.
ABSTRACT Traits associated with meat performance are characterized by a complex inheritance model, including the effects of non-allelic interactions. The aim of this study was to estimate the significance of non-allelic interactions between pairs of loci in each of two alternative groups based on four important performance traits of ducks: body weight (BW), carcass yield calculated as the ratio of carcass weight to body weight (CY), breast and leg muscle yield as a fraction of carcass weight (BL-CW), and skin weight and abdominal fat, again as a fraction of carcass weight (SF-CW). The experimental material was based on an F2 cross between two parental lines: A-55 (Polish Peking) and GL-30 (French Peking). In total, 368 individuals were recorded. All of the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Duck; Categorized traits; Entropy analysis; Microsatellite markers.
Ano: 2020 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2020000200301
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Isolation of 12 microsatellite markers following a pyrosequencing procedure and cross-priming in two invasive cryptic species, Alexandrium catenella (group IV) and A. tamarense (group III) (Dinophyceae) ArchiMer
Laporte, Martin; Shao, Zhaojun; Berrebi, Patrick; Laabir, Mohamed; Abadie, Eric; Faivre, Nicolas; Rieuvilleneuve, Fabien; Masseret, Estelle.
Alexandrium catenella (group IV) and Alexandrium tamarense (group III) (Dinophyceae) are two cryptic invasive phytoplankton species belonging to the A. tamarense species complex. Their worldwide spread is favored by the human activities, transportation and climate change. In order to describe their diversity in the Mediterranean Sea and understand their settlements and maintenances in this area, new microsatellite markers were developed based on Thau lagoon (France) samples of A. catenella and A. tamarense strains. In this study twelve new microsatellite markers are proposed. Five of these microsatellite markers show amplifications on A. tamarense and ten on A. catenella. Three of these 12 microsatellite markers allowed amplifications on both cryptic...
Tipo: Text Palavras-chave: Invasive species; Phytoplankton; Alexandrium; Dinophyceae; Microsatellite markers; Biogeography.
Ano: 2014 URL: http://archimer.ifremer.fr/doc/00189/30026/29398.pdf
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Mapping of quantitative trait loci and confirmation of the FAT1 region on chromosome 4 in an F2 population of pigs Genet. Mol. Biol.
Silva,Kleibe de Moraes; Paixão,Débora Martins; Silva,Priscila Vendramini; Solero,Bruna Pena; Pereira,Mario Sérgio; Lopes,Paulo Sávio; Guimarães,Simone Eliza Facioni.
The objective was to map QTL on porcine chromosome 4 and to associate them with carcass and internal organ traits in an F2 population. The F1 population was produced by outbreed crossing, using two native Brazilian breed Piau boars and 18 commercial sows. A total of 617 F2 animals issued from 11 F1 boars and 54 F1 sows were typed for a total of five microsatellite markers. The data were analyzed by multiple regressions developed for the analysis of crosses between outbred lines, using the QTL Express software. Significant evidence for QTL was found for pig chromosome 4 regarding carcass and internal organ traits. All QTL were detected in the same region of the chromosome, designated FAT1.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Fatness; Pig production; Divergent crosses; Microsatellite markers; QTL mapping.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000300013
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Microsatellite markers as a tool to study reproductive success in the Pacific oyster, Crassostrea gigas (Thunberg), under controlled hatchery conditions ArchiMer
Boudry, Pierre; Collet, Bertrand; Cornette, Florence; Hervouet, Veronique; Bonhomme, Francois.
Oysters, like many marine species have a very high fecundity. Previous studies have shown that populations, from both hatcheries and the natural environment, have very low Ne/N ratios. These observations reveal high variation in reproductive success. In order to study individual reproductive success under controlled conditions, we used microsatellite markers to quantify parental contributions in in vitro crosses (5 males and 5 females) of Crassostrea gigas, the Pacific oyster. High polymorphism of the microsatellites (more that 50 alleles per locus) eased the parentage identifications. The results of a cross allowing gametic competition were compared with the results from a second cross where the gametes of the same parents were kept separate for each...
Tipo: Text Palavras-chave: Genetic; Hatchery; Microsatellite markers; Crassostrea gigas; Pacific oysters.
Ano: 2000 URL: http://archimer.ifremer.fr/doc/2000/acte-3457.pdf
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Mitochondrial and microsatellite genetic differentiation of the flat oyster Ostrea edulis along the european coasts, and comparison with allozyme data ArchiMer
Lapegue, Sylvie; Diaz-almela, E.; Launey, Sophie; Ledu, Christophe; Boudry, Pierre; Naciri-graven, Yamama; Bonhomme, Francois.
Natural populations of the European flat oyster, Ostrea edulis, have been harvested since Roman times. This species is distributed along the European Atlantic coasts, from Norway to Morocco, as well as the Mediterranean Sea and Black sea coasts. Previous studies, based on allozymes, have concluded that isolation by distance is taking place in the Mediterranean sea and the Atlantic ocean. Because of the presumed non neutrality of these markers, another approach was made to examine the genetic differentiation of this species using nuclear microsatellite and mitochondrial markers, both considered as neutral, and to compare the results to those observed with allozymes.
Tipo: Text Palavras-chave: Microsatellite markers; Ostrea edulis; Flat oyster; Genetic.
Ano: 2000 URL: http://archimer.ifremer.fr/doc/2000/acte-3497.pdf
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Phenotypic and molecular characterization of apple (Malus × domestica Borkh) genetic resources in Greece Scientia Agricola
Ganopoulos,Ioannis; Tourvas,Nikolaos; Xanthopoulou,Aliki; Aravanopoulos,Filippos A.; Avramidou,Euaggelia; Zambounis,Antonios; Tsaftaris,Athanasios; Madesis,Panagiotis; Sotiropoulos,Thomas; Koutinas,Nikolaos.
ABSTRACT: The phenotypic and genetic analysis of apple (Malus × domestica Borkh) genotypes is essential for breeding species. Information on the morphology and genetic structure of apple offers significant help for germplasm maintenance and selection of suitable material to breed superior cultivars. This study shows the results of an investigation on the morphology and the genetic diversity for 19 apple cultivars, which are preserved in an ex situ collection in Naoussa, Central Macedonia, Greece. Information was recorded over a 5-year period for 47 traits describing plant morphology and phenotype, as well as leaf and fruit quality. Data were analyzed using the principal component analysis (PCA) method. The Euclidean distance metric and the Ward’s...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Genetic diversity; Genotyping; Microsatellite markers; Multivariate analysis; Phenotyping.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162018000600509
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Population structure and impact of recurrent selection on popcorn using EST-SSR markers Agronomy
Guimarães, Amanda Gonçalves; Amaral Júnior, Antônio Teixeira do; Almeida Filho, Janeo Eustáquio de; Pena, Guilherme Ferreira; Vittorazzi, Cássio; Pereira, Messias Gonzaga.
The success of any recurrent selection program depends on the genetic variability of the evaluated population, which is used to refer to the diversity of existing alleles at many genetic loci. Thus, the goal of the present study was to investigate the impact of recurrent selection across nine cycles of a UENF-14 popcorn population through the analysis of genetic diversity and structure using microsatellite markers (EST-SSRs). Genomic DNA was extracted from young leaves of 25 individuals from each cycle (C0, C1 C2, C3, C4, C5, C6, C7, and C8), totaling 225 samples from the UENF-14 population. Fifty EST-SSR markers were used for the analysis of genetic diversity across the recurrent selection cycles, 16 of which were polymorphic. Thirty-four alleles were...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Zea mays L. var. Everta; Variability; Microsatellite markers; Heterozygosity; Equilibrium.; Melhoramento Vegetal.
Ano: 2018 URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/35218
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Population structure and impact of recurrent selection on popcorn using EST-SSR markers Agronomy
Guimarães, Amanda Gonçalves; Amaral Júnior, Antônio Teixeira do; Almeida Filho, Janeo Eustáquio de; Pena, Guilherme Ferreira; Vittorazzi, Cássio; Pereira, Messias Gonzaga.
The success of any recurrent selection program depends on the genetic variability of the evaluated population, which is used to refer to the diversity of existing alleles at many genetic loci. Thus, the goal of the present study was to investigate the impact of recurrent selection across nine cycles of a UENF-14 popcorn population through the analysis of genetic diversity and structure using microsatellite markers (EST-SSRs). Genomic DNA was extracted from young leaves of 25 individuals from each cycle (C0, C1 C2, C3, C4, C5, C6, C7, and C8), totaling 225 samples from the UENF-14 population. Fifty EST-SSR markers were used for the analysis of genetic diversity across the recurrent selection cycles, 16 of which were polymorphic. Thirty-four alleles were...
Tipo: Info:eu-repo/semantics/article Palavras-chave: 5.01.03.05-9; 2.08.04.00-8 Zea mays L. var. Everta; Variability; Microsatellite markers; Heterozygosity; Equilibrium. Melhoramento Vegetal.
Ano: 2018 URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/35218
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